cDNA Cloning, Tissue Distribution, and Substrate Characteristics of a cis-Retinol/3α-Hydroxysterol Short-chain Dehydrogenase Isozyme

Cloning and expression of a cDNA encoding bovine retinal pigment epithelial 11-cis retinol dehydrogenase.

PURPOSE Identification of a 32-kd protein in the bovine retinal pigment epithelium. METHODS A bovine retinal pigment epithelium cDNA library was constructed in the bacteriophage lambda ZAP Express. A monoclonal antibody, designated 21-C3/AV, was used to isolate the cDNA encoding the 21-C3/AV antigen. A positive full-length clone, designated 21-C3RDH/CD, was sequenced. Northern blot analysis w...

1242 Cloning and expression of a cDNA encoding bovine retinal pigment epithelial 11-cis retinol dehydrogenase

a Tdenzification of a 32 kDaiton protein in the bovine retinal pigment epithelium. Hethods A bovine retinal pigment epithelium cDNA library wa* constructed and immunologically screened with monoclonai antibody 21-C3/AV. A positive full-length clone, 21-C3RDH/CD, wan sequenced. Northern blot analysis were performed to analyze the tissue expression pattern. In order 'co analyze the function of th...

Biochemical properties, tissue expression, and gene structure of a short chain dehydrogenase/ reductase able to catalyze cis-retinol oxidation.

We have identified a retinol dehydrogenase (cRDH) that catalyzes the oxidation of 9-cis- but not all-trans-retinol and proposed that this enzyme plays an important role in synthesis of the transcriptionally active retinoid, 9-cis-retinoic acid. There is little information regarding either the biochemical properties of cRDH or how its 9-cis-retinol substrate is formed. We now report studies of t...

The pea SAD short-chain dehydrogenase/reductase: quinone reduction, tissue distribution, and heterologous expression.

The pea (Pisum sativum) tetrameric short-chain alcohol dehydrogenase-like protein (SAD) family consists of at least three highly similar members (SAD-A, -B, and -C). According to mRNA data, environmental stimuli induce SAD expression. The aim of this study was to characterize the SAD proteins by examining their catalytic function, distribution in pea, and induction in different tissues. In enzy...

pH and Substrate Chain Length Dependence of the Activity of "Short-Chain"-, "Medium-Chain"- and "Long-Chain" -Acyl-CoA Dehydrogenase